Nitrazine Paper Test: A Comprehensive Analysis


The Nitrazine paper test is a valuable diagnostic method used to assess the rupture of fetal membranes during pregnancy. In this article, we will delve into the results of a recent study that examined the efficacy of this test in predicting premature rupture of membranes (PROM) and explore its potential implications for healthcare professionals and expectant mothers.

Participants and Variables

The study included a diverse group of participants, with ages ranging from 20 to 44 years. The majority of subjects were self-employed, while the controls consisted mainly of individuals employed in the public or private sector. Statistical analysis of the socio-demographic and obstetric variables revealed significant differences between the two groups (P < 0.001).

Duration of Liquor Drainage

The duration of liquor drainage before presentation varied among the participants. The majority presented within 24 hours of PROM, while a small percentage presented after 72 hours. Interestingly, those who presented between 48 and 72 hours after the onset of liquor drainage had the highest median level of vaginal fluid urea. However, the duration of PROM did not have a significant effect on the vaginal fluid levels of urea or creatinine (P = 0.829 and P = 0.634, respectively).

Nitrazine Test Results

The study examined the percentage of positive Nitrazine tests in relation to the duration of PROM. It was observed that the likelihood of obtaining a false negative test increased with the duration of liquor drainage. Surprisingly, in cases where the duration of PROM exceeded 72 hours, all tests were truly positive.

Predictive Accuracy of Urea and Creatinine

To determine the predictive accuracy of urea and creatinine in diagnosing PROM, a Receiver Operating Characteristic Curve (ROC) analysis was conducted. The cutoff value for urea was >12.7 mg/dl, with a sensitivity of 19.64% and a specificity of 94.44%. For creatinine, the cutoff value was >0.8 mg/dl, with a sensitivity of 48.21% and a specificity of 65.28%.

Comparison of Tests

A comparison of nitrazine, urea, and creatinine tests was conducted to evaluate their efficacy in predicting PROM. Nitrazine showed the highest percentage of positive results in the study group and was negative in the control group, indicating its reliability in predicting PROM. Creatinine also demonstrated some reliability, albeit to a lesser extent. Urea, on the other hand, exhibited the lowest sensitivity among the three tests.


In conclusion, the Nitrazine paper test, along with the assessment of urea and creatinine levels in vaginal fluid, can provide valuable insights for healthcare professionals in diagnosing PROM. The results of this study highlight the efficacy of the Nitrazine test in accurately identifying PROM cases. However, further research is required to enhance the sensitivity of the urea and creatinine tests. For more information on pregnancy-related topics, visit Quill And Fox, your trusted source for reliable and helpful information.